Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production.
Identifieur interne : 000F53 ( Main/Exploration ); précédent : 000F52; suivant : 000F54Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production.
Auteurs : Melanie L. Lewis Ivey [États-Unis] ; Sally A. MillerSource :
- Mycologia [ 0027-5514 ]
Descripteurs français
- KwdFr :
- Benzothiazoles (composition chimique), Colorants fluorescents (composition chimique), Coloration et marquage (méthodes), Maladies des plantes (microbiologie), Phytophthora (composition chimique), Phytophthora (croissance et développement), Quinoléinium, composés (composition chimique), Spores (composition chimique), Spores (croissance et développement).
- MESH :
- composition chimique : Benzothiazoles, Colorants fluorescents, Phytophthora, Quinoléinium, composés, Spores.
- croissance et développement : Phytophthora, Spores.
- microbiologie : Maladies des plantes.
- méthodes : Coloration et marquage.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Benzothiazoles, Fluorescent Dyes, Quinolinium Compounds.
- chemistry : Phytophthora, Spores.
- growth & development : Phytophthora, Spores.
- methods : Staining and Labeling.
- microbiology : Plant Diseases.
Abstract
The fluorescent vital dye FUN®-1 (2-chloro-4-[2,3-dihydro-3-methyl-{benzo-1,3-thiazol-2-yl}-methylidene]-1-phenylquinolinium iodide) was evaluated as a tool to assess Phytophthora capsici sporangia and zoospore metabolic activity and viability. Under aerobic conditions, mycelia, sporangia and zoospores cultured on agar medium and stained with FUN-1 exhibited red fluorescent cylindrical intravacuolar structures (CIVS) that were clearly visible at 100× magnification. Encysted zoospores did not exhibit CIVS after exposure to FUN-1 dye. Over 7 d there was a significant reduction in the percent of sporangia containing CIVS, which corresponded with a significant increase in zoospore formation and release. The decline in the percentage of metabolically active sporangia and increase in the number of zoospores fit both a linear and log regression model. The FUN-1 dye was suitable for distinguishing between live and dead sporangia and effective in monitoring the change in metabolic activity of sporangia over time. It will be useful in determining parameters, including P. capsici culture age, that maximize production of zoospores in vitro.
DOI: 10.3852/106.2.362
PubMed: 24782503
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<affiliation wicri:level="2"><nlm:affiliation>Ohio State University-Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691.</nlm:affiliation>
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<wicri:cityArea>Ohio State University-Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster</wicri:cityArea>
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<author><name sortKey="Miller, Sally A" sort="Miller, Sally A" uniqKey="Miller S" first="Sally A" last="Miller">Sally A. Miller</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Benzothiazoles (chemistry)</term>
<term>Fluorescent Dyes (chemistry)</term>
<term>Phytophthora (chemistry)</term>
<term>Phytophthora (growth & development)</term>
<term>Plant Diseases (microbiology)</term>
<term>Quinolinium Compounds (chemistry)</term>
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<term>Spores (growth & development)</term>
<term>Staining and Labeling (methods)</term>
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<term>Maladies des plantes (microbiologie)</term>
<term>Phytophthora (composition chimique)</term>
<term>Phytophthora (croissance et développement)</term>
<term>Quinoléinium, composés (composition chimique)</term>
<term>Spores (composition chimique)</term>
<term>Spores (croissance et développement)</term>
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<term>Fluorescent Dyes</term>
<term>Quinolinium Compounds</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phytophthora</term>
<term>Spores</term>
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<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Benzothiazoles</term>
<term>Colorants fluorescents</term>
<term>Phytophthora</term>
<term>Quinoléinium, composés</term>
<term>Spores</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term>
<term>Spores</term>
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<term>Spores</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term>
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<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Coloration et marquage</term>
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<front><div type="abstract" xml:lang="en">The fluorescent vital dye FUN®-1 (2-chloro-4-[2,3-dihydro-3-methyl-{benzo-1,3-thiazol-2-yl}-methylidene]-1-phenylquinolinium iodide) was evaluated as a tool to assess Phytophthora capsici sporangia and zoospore metabolic activity and viability. Under aerobic conditions, mycelia, sporangia and zoospores cultured on agar medium and stained with FUN-1 exhibited red fluorescent cylindrical intravacuolar structures (CIVS) that were clearly visible at 100× magnification. Encysted zoospores did not exhibit CIVS after exposure to FUN-1 dye. Over 7 d there was a significant reduction in the percent of sporangia containing CIVS, which corresponded with a significant increase in zoospore formation and release. The decline in the percentage of metabolically active sporangia and increase in the number of zoospores fit both a linear and log regression model. The FUN-1 dye was suitable for distinguishing between live and dead sporangia and effective in monitoring the change in metabolic activity of sporangia over time. It will be useful in determining parameters, including P. capsici culture age, that maximize production of zoospores in vitro. </div>
</front>
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<DateCompleted><Year>2014</Year>
<Month>07</Month>
<Day>15</Day>
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<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
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<JournalIssue CitedMedium="Print"><Volume>106</Volume>
<Issue>2</Issue>
<PubDate><MedlineDate>2014 Mar-Apr</MedlineDate>
</PubDate>
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<Title>Mycologia</Title>
<ISOAbbreviation>Mycologia</ISOAbbreviation>
</Journal>
<ArticleTitle>Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production.</ArticleTitle>
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<Abstract><AbstractText>The fluorescent vital dye FUN®-1 (2-chloro-4-[2,3-dihydro-3-methyl-{benzo-1,3-thiazol-2-yl}-methylidene]-1-phenylquinolinium iodide) was evaluated as a tool to assess Phytophthora capsici sporangia and zoospore metabolic activity and viability. Under aerobic conditions, mycelia, sporangia and zoospores cultured on agar medium and stained with FUN-1 exhibited red fluorescent cylindrical intravacuolar structures (CIVS) that were clearly visible at 100× magnification. Encysted zoospores did not exhibit CIVS after exposure to FUN-1 dye. Over 7 d there was a significant reduction in the percent of sporangia containing CIVS, which corresponded with a significant increase in zoospore formation and release. The decline in the percentage of metabolically active sporangia and increase in the number of zoospores fit both a linear and log regression model. The FUN-1 dye was suitable for distinguishing between live and dead sporangia and effective in monitoring the change in metabolic activity of sporangia over time. It will be useful in determining parameters, including P. capsici culture age, that maximize production of zoospores in vitro. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lewis Ivey</LastName>
<ForeName>Melanie L</ForeName>
<Initials>ML</Initials>
<AffiliationInfo><Affiliation>Ohio State University-Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691.</Affiliation>
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<ForeName>Sally A</ForeName>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D052160" MajorTopicYN="N">Benzothiazoles</DescriptorName>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">cylindrical intravacuolar structures (CIVS)</Keyword>
<Keyword MajorTopicYN="N">encysted zoospores</Keyword>
<Keyword MajorTopicYN="N">sporangia</Keyword>
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